A simple, novel, sensitive, and specific spectrophotometric method was developed and validated for the determination of Metoprolol succinate and Clopidogrel bisulphate in tablet dosage form. First order derivative spectroscopy method is adopted to eliminate spectral interference. The method obeys Beer�s Law in concentration ranges selected for evaluation. Metoprolol succinate and Clopidogrel bisulphate have ?max at 223nm and 222.22 nm respectively in 0.1N HCL solvent. The method was validated for linearity, accuracy and precision as per ICH guidelines. The zero crossing point for Metoprolol succinte and Clopidogrel bisulphate was 245.76nm and 276.13nm respectively. The LOD and LOQ value were found to be 0.15 and 0.18 ?g/ml for Metoprolol succinate and 2 and 4.5 ?g/ml for Clopidogrel bisulphate respectively. The developed and validated method was successfully used for the quantitative analysis of combined tablet dosage form.
Loading....